H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate)

H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate)
  • CAS No.:4091-99-0
  • Grade:95%
  • Formula:C24H16Cl2O7
  • M.W.:487.3
  • Solvent:DMF, DMSO, Ethanol

Important Notice

  1. This product is a chemical product/pharmaceutical intermediate. It is not a drug, an active pharmaceutical ingredient (API), or a pharmaceutical-grade material. It has not been granted a drug approval number or a Chemical Drug Substance Approval Notice, has not passed drug-related review and approval, and is not manufactured, tested, or released in accordance with pharmaceutical GMP.
  2. This product is intended solely for non-clinical research, process development, quality studies, further chemical processing, or chemical synthesis. It must not be used in drug-product manufacturing, clinical trials, clinical diagnosis, or administration to humans or animals, or be sold, used as an input, submitted for regulatory filing, or released as an API or pharmaceutical material.
  3. The purchaser must not use this product, or supply it to another party, for personal consumption, sports or fitness, weight loss, health supplements, food, or animal feed.

Overview

H2DCFDA is a cell-permeant indicator used to detect reactive oxygen species (ROS) in living cells. Initially non-fluorescent, it becomes highly fluorescent after intracellular esterases remove its acetate groups and oxidation occurs. The resulting product is 2’,7’-dichlorofluorescein (DCF), which emits bright green fluorescence with an absorption maximum at 511 nm and a fluorescence maximum at 533 nm. It’s commonly employed for ROS assays in living cells and is not suitable for sample fixation.

Application

Products > Fluorescent Dye > Cell staining

Product Information

  • Catalog No.: BP-42033
  • CAS No.: 4091-99-0
  • Molecular Formula: C24H16Cl2O7
  • Molecular Weight: 487.3
  • Purity: 95%
  • Storage: -20°C
  • Solubility: DMF, DMSO, Ethanol
  • Availability: 24 Hours

Source

Original product source


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