H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate)

Overview

H2DCFDA is a cell-permeant indicator used to detect reactive oxygen species (ROS) in living cells. Initially non-fluorescent, it becomes highly fluorescent after intracellular esterases remove its acetate groups and oxidation occurs. The resulting product is 2’,7’-dichlorofluorescein (DCF), which emits bright green fluorescence with an absorption maximum at 511 nm and a fluorescence maximum at 533 nm. It’s commonly employed for ROS assays in living cells and is not suitable for sample fixation.

Application

Products > Fluorescent Dye > Cell staining

Product Information

• Catalog No.: BP-42033
• CAS No.: 4091-99-0
• Molecular Formula: C24H16Cl2O7
• Molecular Weight: 487.3
• Purity: 95%
• Storage: -20°C
• Solubility: DMF, DMSO, Ethanol
• Availability: 24 Hours

Source

Original product source