3-Oxo-bufalin is an active compound that can be isolated from cultured toad liver tissue. 3-Oxo-bufalin can be produced through a metabolic pathway, and it is a metabolite of Bufalin ( HY-N0877 ) catalyzed by the stereoselective 3β-dehydrogenase. Bufalin is a Na+/K+-ATPase inhibitor and has anti-tumor activity.
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