2-Thiocytidine is a natural thionucleoside in prokaryotic tRNA, which is biosynthesized under the catalysis of intracellular sulfur transferase systems and possesses multiple activities such as metal coordination and nucleic acid conformation regulation. 2-Thiocytidine forms stable Zn 2+ and Cd 2+ complexes via its (C2) S group, with partial involvement of N3; metal binding acidifies the deprotonation process of (C4) NH2. 2-Thiocytidine can be used in related research in various fields including nanotechnology and click chemistry for interstrand DNA crosslinking.
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