Aniline blue diammonium is a component of commonly used polychrome stains. Aniline blue diammonium is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Aniline blue diammonium is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.
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