4-Pyridoxolactone (β-Pyracin) is a critical substrate in vitamin B6 degradation pathway I, primarily involved in the vitamin B6 metabolic process mediated by soil microorganisms . 4-Pyridoxolactone serves as the specific substrate for 4-pyridoxolactonase, undergoing a zinc-dependent lactone-ring hydrolysis reaction catalyzed by this enzyme to generate 4-pyridoxic acid (4PA).
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