Autophagy-IN-9 is a ATG5 inhibitor with an IC 50 of 21.29 μM against ATG5-TECAIR . Autophagy-IN-9 blocks the binding of ATG5 to the hot-spot amino acid residues of TECAIR protein, disrupts the assembly of ATG5-related ternary complexes, inhibits autophagosome formation, and thereby downregulates cellular autophagy levels. Autophagy-IN-9 can be used in autophagy-related research, such as studies on infection .
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