Trisaccharides 3 (compoudn 3) is a GlfT2 acceptor substrate with a 50 μM K a . Trisaccharides 3 acts as an acceptor substrate for the β-(1→6) galactofuranosyl transferase activity of GlfT2 , binds competitively at GlfT2 ’s single active site pocket, and undergoes galactosylation by GlfT2 with UDP-Galf to form a tetrasaccharide product. Trisaccharides 3 can be used for the research of tuberculosis, hiv-associated Mycobacterium avium infections.
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