Mito-Cu (II) is a mitochondria-targeted fluorescent probe (Ex/Em = 370/450 nM). Mito-Cu (II) specifically accumulates in mitochondria of living cells and enables real-time detection of exogenous Cu 2+ within mitochondria of living cells. Mito-Cu (II) achieves "on-off-on" fluorescence switching through sequential exposure to Cu 2+ and ethylenediaminetetraacetic acid (EDTA) ( HY-Y0682 ). Its fluorescence is quenched after forming a 1:1 complex with Cu 2+ , and the fluorescence recovers when Cu 2+ is chelated by EDTA.
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