BI dihydrochloride is a DFHBI ( HY-110250 ) derivative for imaging of RNA in cells. BI dihydrochloride increases brightness and photostability of Broccoli aptamers in cells. BI dihydrochloride enables imaging of single Broccoli-tagged mRNAs in living cells. BI dihydrochloride exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli.
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