CycLuc1 methyl ester (Example 1) is a selective CES1 substrate and indirect bioluminescent inducer. CycLuc1 methyl ester undergoes catalytic hydrolysis via CES1 -mediated ester bond cleavage to form a carboxyl product. The carboxyl product of CycLuc1 methyl ester undergoes an oxidation reaction with luciferase to generate a detectable bioluminescent signal. CycLuc1 methyl ester enables quantitative detection of CES1 activity.
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