H-Ala-pNA is an L-amino acid p-nitroaniline ( pNA ) derivative and a specific substrate for leukotriene A4 hydrolase . The D-enantiomer of H-Ala-pNA shows no activity toward leukotriene A4 hydrolase . H-Ala-pNA can be catalytically hydrolyzed by leukotriene A4 hydrolase , and the p-nitroaniline produced during the reaction is monitored spectrophotometrically at 405 nm to enable quantitative detection of enzyme activity. H-Ala-pNA is used to evaluate the potency of inhibitors targeting the amidase activity of leukotriene A4 hydrolase .
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