Lyngbyatoxin B is a complex compound in a versatile biosynthetic pathway from the marine cyanobacterium Moorea producens. By heterologously expressing the lyngbyatoxin biosynthetic pathway in Escherichia coli, lyngbyatoxin A (25.6 mg/L) and its precursor indolactam-V (150 mg/L) were successfully produced. This approach confirmed the biosynthetic route of lyngbyatoxin and laid a chemical foundation for future pathway engineering. In addition, the successful production of lyngbyatoxin A in E. coli opens the possibility for heterologous expression and characterization of other cyanobacterial natural products.
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