Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a K d of 2 μM. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation ( LLPS ). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations.
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