Hoechst 33342

Overview

Hoechst 33342 is a blue-emitting fluorescent dye which selectively binds to adenine-thymine-rich regions in double-stranded DNA. With excitation/emission maxima at 351/461 nm, it exhibits a considerable Stokes shift. Notably, Hoechst 33342 is less toxic than DAPI, ensuring better cell viability. It is widely used in fluorescence microscopy and flow cytometry for staining chromosomes and nuclei in live and fixed cells, particularly to distinguish condensed pycnotic nuclei in apoptotic cells and for cell sorting.

Application

Products > Fluorescent Dye > Nucleic acid stain | Products > Fluorescent Dye > Nucleic acid stain > Blue Excitation

Product Information

• Catalog No.: BP-42012
• CAS No.: 23491-52-3
• Molecular Formula: C27H28N6O
• Molecular Weight: 452.6
• Purity: 95%
• Storage: -20°C
• Solubility: Water, DMSO, DMF
• Availability: In Stock

Source

Original product source