Hoechst 34580

Overview

Hoechst 34580 is a blue-emitting fluorescent dye that selectively binds to adenine-thymine-rich regions in double-stranded DNA. It exhibits excitation/emission peaks at 380/438 nm when bound to DNA, with fluorescence intensity sensitive to solvent pH. Unbound dye emits green light in the 510–540 nm range, more pronounced at higher concentrations or inadequate washing. While capable of penetrating living cells, it is less permeable than Hoechst 33342. Importantly, the dye is less toxic than DAPI, ensuring better cell viability post-staining.

Application

Products > Fluorescent Dye > Nucleic acid stain | Products > Fluorescent Dye > Nucleic acid stain > Blue Excitation

Product Information

• Catalog No.: BP-42013
• CAS No.: 911004-45-0
• Molecular Formula: C27H29N7
• Molecular Weight: 451.6
• Purity: 95%
• Storage: -20°C
• Solubility: Water, DMSO, DMF
• Availability: 24 Hours

Source

Original product source