Resorufin-β-D-galactopyranoside is a commonly used substrate in various biochemical assays to measure the activity of β-galactosidase, an important enzyme involved in lactose metabolism and regulation of gene expression. Resorufin-β-D-galactopyranoside has unique chemical properties and can be hydrolyzed by β-galactosidase to form a red fluorescent product called resorufin. This makes it a useful tool for detecting and quantifying β-galactosidase activity in biological samples such as bacteria or mammalian cells.
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