5-Bromo-3-indolyl β-D-galactopyranoside

Overview

5-Bromo-3-indolyl β-D-galactopyranoside (Bluo-Gal) is a chromogenic substrate for β-galactosidase . 5-Bromo-3-indolyl β-D-galactopyranoside is hydrolyzed by the enzyme to generate a 5-bromoindole intermediate, which is further oxidized to form an insoluble blue precipitate. 5-Bromo-3-indolyl β-D-galactopyranoside can specifically recognize bacterial β-galactosidases (such as the product of the Escherichia coli lacZ gene) and reacts at pH 7.4, making it suitable for light and electron microscopic observations. 5-Bromo-3-indolyl β-D-galactopyranoside can be used in histochemical detection of reporter gene expression in transgenic organisms, such as the localization analysis of β-galactosidase activity in mouse embryos or muscle tissues.

Application

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Product Information

• Catalog No.: HY-137276
• CAS No.: 97753-82-7
• Molecular Formula: C14H16BrNO6
• Molecular Weight: 374.18
• Purity: 99.89%
• Storage: 4°C, protect from light * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
• Availability: In-stock

Source

Original product source