BGN3 is a good substrate for the SNAP- tag ? and H 5 enzymes . The activities of SNAP- tag ? and H 5 enzymes on BGN3 are reasonable (IC 50 = 15.6 and 23.5 μM, respectively). BGN3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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