TFMU-ADPr

Overview

TFMU-ADPr is a selective reporter substrate of SARS-CoV-2 Macro1 ( IC 50 =0.59 μM), with an excitation wavelength ( λ Ex ) of 385 nm, and an emission wavelength ( λ Em ) of 502 nm (or 495 nm). TFMU-ADPr can also undergo enzymatic hydrolysis with Poly(ADP-ribose) Glycohydrolase (PARG) sourced from human, Tetrahymena thermophila and ADP-ribosylhydrolase 3 from human to release fluorophores, thereby directly reporting total poly (ADP-ribose) hydrolase activity. TFMU-ADPr binds to the ADPr-binding site of SARS-CoV-2 Macro1, and its TFMU moiety inserts into the narrow hydrophobic groove of this protein. TFMU-ADPr can thus be used to evaluate small-molecule inhibitors targeting PAR hydrolases under in vitro conditions, to investigate the regulatory mechanisms of ADP-ribosyl catabolic enzymes , or to detect PAR hydrolase activity in whole-cell lysate assays. TFMU-ADPr is also applicable to COVID-19 -related research.

Application

Products | Products > Disease Areas > SARS-CoV-2 Infection | Products > Disease Areas > Infection | Products > Disease Areas > Coronavirus Infection | Products > Signaling Pathways > Anti-infection | Products > Signaling Pathways > Cell Cycle/DNA Damage | Products > Signaling Pathways > GPCR/G Protein | Products > Research Areas > Infection | Products > Signaling Pathways > SARS-CoV | Products > Signaling Pathways > Poly(ADP-ribose) Glycohydrolase (PARG) | Products > Signaling Pathways > Protease Activated Receptor (PAR)

Product Information

• Catalog No.: HY-146248
• CAS No.: 2412923-11-4
• Molecular Formula: C25H26F3N5O16P2
• Molecular Weight: 771.44
• Purity: 99.98%
• Storage: Please store the product under the recommended conditions in the Certificate of Analysis.

Source

Original product source