D-Val-Leu-Lys-pNA (S-2251) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen . D-Val-Leu-Lys-pNA acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.
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