RdRP-IN-9 (Compound 6b) is a reversible covalent allosteric inhibitor of RdRp . RdRP-IN-9 demonstrates high anti-coronavirus activity, with an EC 50 value of 0.68 μM against HCoV-OC43 RdRP-IN-9 exerts a more prolonged antiviral effect by reversibly acylating Cys12 in the NiRAN domain of non-structural protein 12 ( nsp 12 ) and exhibits synergistic anti-coronavirus activity with Molnupiravir ( HY-135853 ) .
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