CHIKV nsP2 protease-IN-2 (Compound 2o) is a allosteric nonstructural protein 2 helicase (nsP2hel) inhibitor with IC 50 s of 0.5 μM and 0.9 μM for nsP2 ATPase and RNA unwindase, respectively. CHIKV nsP2 protease-IN-2 has broad-spectrum antialphaviral activity against chikungunya virus (CHIKV), Mayaro virus (MAYV), and Venezuelan equine encephalitis virus (VEEV) ( EC 50 of 120 nM for CHIKV-nLuc). CHIKV nsP2 protease-IN-2 can be used for alphaviruses infections research.
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