3'-O-(2-Nitrobenzyl)-dTTP

Overview

3'-O-(2-Nitrobenzyl)-dTTP acts as a reversible terminator for DNA chain elongation. 3'-O-(2-Nitrobenzyl)-dTTP interacts with 9°N polymerase (exo-) A485L/Y409V, gets recognized and incorporated into the elongating DNA chain, thereby temporarily terminating the polymerase reaction. 3'-O-(2-Nitrobenzyl)-dTTP carries a 3'-O-(2-nitrobenzyl) blocking group, which can be removed by laser irradiation to regenerate the free 3'-OH − group and restore polymerase activity. 3'-O-(2-Nitrobenzyl)-dTTP is applicable in DNA sequencing studies.

Application

Products | Products > Disease Areas > Others | Products > Signaling Pathways > Cell Cycle/DNA Damage | Products > Research Areas > Others | Products > Signaling Pathways > DNA/RNA Synthesis

Product Information

• Catalog No.: HY-185274
• CAS No.: 219607-49-5
• Molecular Formula: C17H22N3O16P3
• Molecular Weight: 617.29
• Purity: 99.98%
• Storage: Please store the product under the recommended conditions in the Certificate of Analysis.

Source

Original product source