Biocytin is a conjugate of D -biotin and L -lysine, where the carboxylate of D -biotin is coupled with the -amine of L -lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays.
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