DiSC3(5) is a fluorescent probe commonly used as a tracer dye to evaluate mitochondrial membrane potential. The excitation/emission wavelength of DiSC3(5) is up to 622/670 nm. DiSC3(5) can inhibit the respiratory system associated with mitochondrial NAD, and the IC 50 value is 8 μM. DiSC3(5) in the presence of Na + /K + -ATPase inhibitor ouabain 2 can induce membrane hyperpolarization of Ehrlich ascites tumor cells.
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