Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca 2+ , Mg 2+ , Sr 2+ , Ba 2+ , K + , and Na + , and its binding affinity for Ca 2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca 2+ in cells.
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