YO-PRO-1 is a non-cell-membrane-penetrating green fluorescent dye . YO-PRO-1 cannot penetrate normal cell membranes but can penetrate the membranes of apoptotic and necrotic cells. YO-PRO-1 has a high affinity for DNA. When it is not bound to DNA, it emits almost no fluorescence, and when it binds to DNA, it can emit green fluorescence (Ex/Em: 488/530±30 nM). YO-PRO-1 is often used together with Propidium Iodide ( HY-D0815 ) to analyze and identify apoptotic and necrotic cells.
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