HBC (HBC 530) is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC has a low fluorescence background, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence ( K d of ~3.5 nM). HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA (Em/Ex = 530/485 nm).
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