BTD probe-1 is a benzothiazine-based chemoproteomic probe and selective protein S-sulfenic acid (Cys-SOH) labeling agent. BTD probe-1 labels protein S-sulfenic acids in vitro in cell and tissue samples, and in situ in intact cells, enabling detection or enrichment of modified proteins/peptides. BTD probe-1 exhibits no cytotoxicity in cells at concentrations ≤1 mM. BTD probe-1 enables global, site-specific mapping and quantification of cysteine S-sulfenylation in complex proteomes with lower input material.
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