NBzF-BG is a novel H 2 O 2 -specific fluorescent probe. NBzF-BG shows visible absorption centered at 505 nm and weak fluorescence with an emission maximum at 525 nm. NBzF-BG is covalently and selectively conjugated with the SNAP-tag protein, leading to formation of the fluorophore-protein conjugate (SNAP-NBzF). SNAP-NBzF rapidly reacts with H 2 O 2 and thereby shows an enhancement in fluorescence.
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