(-)-Phaselic acid is a protein protectant that can be isolated from red clover ( Trifolium pratense ) leaves. (-)-Phaselic acid is synthesized through an acyl transfer reaction between caffeoyl-CoA and malic acid catalyzed by the HCT2 enzyme. As a substrate for endogenous polyphenol oxidase (PPO), its oxidation to o-quinone can inhibit protein degradation during forage storage. (-)-Phaselic acid can be used to improve the protein preservation performance of forage crops such as alfalfa, reducing nutritional losses during storage and environmental nitrogen pollution.
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