Biotin-NAD + is a PARylation donor and also a substrate of poly (ADP-ribose) polymerase-1 ( PARP1 ). Under the mediation of PARP1 , Biotin-NAD + undergoes poly (ADP-ribosyl) ation to generate biotinylated poly (ADP-ribose) polymers . Biotin-NAD + can be used for pull-down assays of PARylated proteins in renal epithelial cells. Biotin-NAD + is applicable to cancer-related research.
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